ARG59200
anti-MED13 antibody
anti-MED13 antibody for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MED13 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MED13 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to L61-Y240 of Human MED13. |
| 偶联标记 | Un-conjugated |
| 別名 | Activator-recruited cofactor 250 kDa component; THRAP1; Thyroid hormone receptor-associated protein 1; Trap240; HSPC221; TRAP240; Mediator complex subunit 13; ARC250; Mediator of RNA polymerase II transcription subunit 13; Vitamin D3 receptor-interacting protein complex component DRIP250; Thyroid hormone receptor-associated protein complex 240 kDa component; DRIP250 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9UHV7 Human Mediator of RNA polymerase II transcription subunit 13 |
|---|---|
| 基因名称 | MED13 |
| 全名 | mediator complex subunit 13 |
| 背景介绍 | This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 239 kDa |
检测图片 (3) Click the Picture to Zoom In
-
ARG59200 anti-MED13 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG59200 anti-MED13 antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
-
ARG59200 anti-MED13 antibody WB image
Western blot: 50 µg of samples under reducing conditions. U2OS and MCF-7 whole cell lysates stained with ARG59200 anti-MED13 antibody at 0.5 µg/ml, overnight at 4°C.
-
ARG59200 anti-MED13 antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59200 anti-MED13 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
