ARG55387
anti-MED12 antibody
anti-MED12 antibody for Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MED12 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MED12 |
| 抗原物种 | Mouse |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 1991-2025 (C-terminus) of Mouse MED12. |
| 偶联标记 | Un-conjugated |
| 別名 | ARC240; OKS; TNRC11; Thyroid hormone receptor-associated protein complex 230 kDa component; TRAP230; OPA-containing protein; OPA1; Mediator complex subunit 12; MED12S; FGS1; CAGH45; Mediator of RNA polymerase II transcription subunit 12; Activator-recruited cofactor 240 kDa component; Trinucleotide repeat-containing gene 11 protein; OHDOX; HOPA; CAG repeat protein 45; Trap230 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # A2AGH6 Mouse Mediator of RNA polymerase II transcription subunit 12 Swiss-port # Q93074 Human Mediator of RNA polymerase II transcription subunit 12 |
|---|---|
| 基因名称 | Med12 |
| 全名 | mediator complex subunit 12 |
| 背景介绍 | The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009] |
| 生物功能 | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity). [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 243 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG55387 anti-MED12 antibody WB image
Western blot: 20 µg of HeLa whole cell lysate stained with ARG55387 anti-MED12 antibody at 1:2000 dilution.
