ARG54064
anti-MECT1 / TORC1 antibody
anti-MECT1 / TORC1 antibody for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes MECT1 / TORC1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | MECT1 / TORC1 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human MECT1 / Torc1 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | CREB-regulated transcription coactivator 1; MECT1; WAMTP1; Transducer of CREB protein 1; TORC1; Mucoepidermoid carcinoma translocated protein 1; Transducer of regulated cAMP response element-binding protein 1; TORC-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 78 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q6UUV9 Human CREB-regulated transcription coactivator 1 |
|---|---|
| 基因名称 | CRTC1 |
| 全名 | CREB regulated transcription coactivator 1 |
| 背景介绍 | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses (By similarity) |
| 生物功能 | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus |
| 研究领域 | Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 67 kDa |
| 翻译后修饰 | Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1 (By similarity). |
检测图片 (4) Click the Picture to Zoom In
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ARG54064 anti-MECT1 / TORC1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54064 anti-MECT1 / TORC1 antibody at 1:300 dilution.
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ARG54064 anti-MECT1 / TORC1 antibody FACS image
Flow Cytometry: K562 cells stained with ARG54064 anti-MECT1 / TORC1 antibody at 1:100 dilution (right) or isotype control antibody (left), followed by incubation with FITC labelled secondary antibody.
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ARG54064 anti-MECT1 / TORC1 antibody WB image
Western blot: HeLa cell lysate stained with ARG54064 anti-MECT1 / TORC1 antibody at 1:1000 dilution.
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ARG54064 anti-MECT1 / TORC1 antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54064 anti-MECT1 / TORC1 antibody.
