ARG66931

anti-MDM2 antibody [SQab22259]

anti-MDM2 antibody [SQab22259] for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody

概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab22259] recognizes MDM2
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab22259
同位型 IgG
靶点名称 MDM2
抗原物种 Human
抗原 Synthetic peptide within Human MDM2.
偶联标记 Un-conjugated
Protein Full name E3 ubiquitin-protein ligase Mdm2
別名 EC 6.3.2.-; Double minute 2 protein; p53-binding protein Mdm2; hdm2; Oncoprotein Mdm2; HDMX; ACTFS; E3 ubiquitin-protein ligase Mdm2; Hdm2

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human dedifferentiated liposarcoma tissue

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05%BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4193 Human MDM2

Swiss-port # Q00987 Human E3 ubiquitin-protein ligase Mdm2

基因名称 MDM2
全名 MDM2 proto-oncogene, E3 ubiquitin protein ligase
背景介绍 This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues
生物功能 E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. [UniProt]
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
预测分子量 55 kDa
翻译后修饰 Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation.
Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization.

检测图片 (1) Click the Picture to Zoom In

  • ARG66931 anti-MDM2 antibody [SQab22259] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human dedifferentiated liposarcoma tissue stained with ARG66931 anti-MDM2 antibody [SQab22259]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).