ARG45128
anti-MDC1 antibody
anti-MDC1 antibody for Flow cytometry,Immunocytochemistry,IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MDC1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MDC1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human MDC1. |
| 偶联标记 | Un-conjugated |
| 別名 | MDC1; mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; Nuclear factor with BRCT domains 1; MDC1; KIAA0170; NFBD1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14676 Human Mediator of DNA damage checkpoint protein 1 |
|---|---|
| 基因名称 | MDC1 |
| 全名 | mediator of DNA damage checkpoint 1 |
| 背景介绍 | The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008] |
| 生物功能 | Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. [UniProt] |
| 细胞定位 | Nucleus; Chromosome. [UniProt] |
| 预测分子量 | 226 kDa |
| 翻译后修饰 | Acetylation; Isopeptide bond; Methylation; Ubl conjugationPhosphorylation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG45128 anti-MDC1 antibody IHC-P image
Immunohistochemistry: Human colon cancer stained with ARG45128 anti-MDC1 antibody at 1 μg/ml dilution.
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ARG45128 anti-MDC1 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45128 anti-MDC1 antibody at 2 ug/ml dilution.
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ARG45128 anti-MDC1 antibody FACS image
Flow Cytometry: 293T stained with ARG45128 anti-MDC1 antibody at 1 µg/10^6 cells dilution.
