ARG41345
anti-MCU antibody
anti-MCU antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MCU |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MCU |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-351 of Human MCU (NP_612366.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Calcium uniporter protein, mitochondrial; CCDC109A; C10orf42; Coiled-coil domain-containing protein 109A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse kidney | ||||||
| 实际分子量 | 33 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q3UMR5 Mouse Calcium uniporter protein, mitochondrial Swiss-port # Q8NE86 Human Calcium uniporter protein, mitochondrial |
|---|---|
| 基因名称 | MCU |
| 全名 | mitochondrial calcium uniporter |
| 背景介绍 | This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012] |
| 生物功能 | Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. [UniProt] |
| 细胞定位 | Mitochondrion inner membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Phosphorylation by CaMK2 in heart leads to increased MCU current (PubMed:23051746, PubMed:25254481). The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results (PubMed:25254480). Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization (PubMed:24800979). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41345 anti-MCU antibody WB image
Western blot: 25 µg of Mouse kidney lysate stained with ARG41345 anti-MCU antibody at 1:1000 dilution.
