ARG66020

anti-MCP5 / Mast cell chymase antibody (Biotin)

anti-MCP5 / Mast cell chymase antibody (Biotin) for ELISA,Western blot and Mouse

概述

产品描述 Biotin-conjugated Rabbit Polyclonal antibody recognizes MCP5 / Mast cell chymase
反应物种 Ms
应用 ELISA, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 MCP5 / Mast cell chymase
抗原物种 Mouse
抗原 E. coli derived recombinant Mouse MCP5.
(GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG)
偶联标记 Biotin
別名 Chymase; Alpha-chymase; Mast cell protease I; chymase; CYH; EC 3.4.21.39; MCT1

应用说明

应用建议
应用 推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66019 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 17228 Mouse CMA1

Swiss-port # P21844 Mouse Chymase

基因名称 Cma1
全名 chymase 1, mast cell
背景介绍 This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015]
生物功能 Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. [UniProt]
预测分子量 27 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Mouse MCP-5 stained with ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin), under non-reducing conditions.

  • ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) standard curve image

    Direct ELISA: ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Mouse MCP-5 stained with ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin), under reducing conditions.

  • ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66019 anti-MCP5 antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.