概述

产品描述	Rabbit Polyclonal antibody recognizes MCM7
反应物种	Hu
应用	WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	MCM7
抗原物种	Human
抗原	Synthetic peptide derived from Human MCM7
偶联标记	Un-conjugated
別名	PNAS146; CDC47 homolog; DNA replication licensing factor MCM7; MCM2; P1.1-MCM3; EC 3.6.4.12; CDC47; P1CDC47; P85MCM; PPP1R104

应用说明

应用建议	应用 推荐稀释比 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量	81kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4176 Human MCM7 Swiss-port # P33993 Human DNA replication licensing factor MCM7
基因名称	MCM7
全名	minichromosome maintenance complex component 7
背景介绍	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
生物功能	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]
细胞定位	Nucleus. [UniProt]
预测分子量	81 kDa
翻译后修饰	O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt]

检测图片 (1) Click the Picture to Zoom In