ARG42812
anti-MCM2 phospho (Ser108) antibody
anti-MCM2 phospho (Ser108) antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MCM2 phospho (Ser108) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MCM2 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Ser108 of Human MCM2. |
| 偶联标记 | Un-conjugated |
| 別名 | CCNL1; Nuclear protein BM28; BM28; DNA replication licensing factor MCM2; cdc19; Minichromosome maintenance protein 2 homolog; MITOTIN; CDCL1; D3S3194; EC 3.6.4.12 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | K562 | ||||||||
| 实际分子量 | ~ 115 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P49736 Human DNA replication licensing factor MCM2 Swiss-port # P97310 Mouse DNA replication licensing factor MCM2 |
|---|---|
| 基因名称 | MCM2 |
| 全名 | minichromosome maintenance complex component 2 |
| 背景介绍 | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] |
| 生物功能 | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 102 kDa |
| 翻译后修饰 | Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG42812 anti-MCM2 phospho (Ser108) antibody WB image
Western blot: K562 cell lysate stained with ARG42812 anti-MCM2 phospho (Ser108) antibody at 1:1000 dilution.
