ARG55381

Name: anti-MCL1 antibody
Brand: arigo Biolaboratories
SKU: ARG55381
Price: 399 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-MCL1 antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399
350

50 μl

279
246

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes MCL1
反应物种	Hu, Ms, Rat
应用	FACS, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	MCL1
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 191-226 of Human MCL1.
偶联标记	Un-conjugated
別名	Bcl-2-related protein EAT/mcl1; BCL2L3; MCL1S; Induced myeloid leukemia cell differentiation protein Mcl-1; MCL1-ES; mcl1/EAT; Bcl-2-like protein 3; EAT; Mcl-1; bcl2-L-3; Bcl2-L-3; MCL1L; TM

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
IHC-P	1:10 - 1:50
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 17210 Mouse MCL1 GeneID: 4170 Human MCL1 GeneID: 60430 Rat MCL1 Swiss-port # P97287 Mouse Induced myeloid leukemia cell differentiation protein Mcl-1 homolog Swiss-port # Q07820 Human Induced myeloid leukemia cell differentiation protein Mcl-1 Swiss-port # Q9Z1P3 Rat Induced myeloid leukemia cell differentiation protein Mcl-1 homolog More
基因名称	MCL1
全名	myeloid cell leukemia 1
背景介绍	This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
生物功能	Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. [UniProt]
细胞定位	Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
预测分子量	37 kDa
翻译后修饰	Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.

检测图片 (4) Click the Picture to Zoom In

ARG55381 anti-MCL1 antibody WB image

Western blot: 30 µg of 293T and HCT116 cell lysates stained with ARG55381 anti-MCL1 antibody at 1:500 dilution.
ARG55381 anti-MCL1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG55381 anti-MCL1 antibody.
ARG55381 anti-MCL1 antibody WB image

Western blot: 20 µg of Rat heart lysate stained with ARG55381 anti-MCL1 antibody at 1:2000 dilution.
ARG55381 anti-MCL1 antibody FACS image

Flow Cytometry: ZR-75-1 cells stained with ARG55381 anti-MCL1 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.