ARG41998
anti-MBD1 antibody
anti-MBD1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
概述
产品描述 | Rabbit Polyclonal antibody recognizes MBD1 |
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反应物种 | Hu |
应用 | ICC/IF, IHC-P, IP, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | MBD1 |
抗原物种 | Human |
抗原 | Synthetic peptide of Human MBD1. |
偶联标记 | Un-conjugated |
別名 | RFT; PCM1; CXXC-type zinc finger protein 3; CXXC3; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1 |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
阳性对照 | HeLa | ||||||||||
实际分子量 | ~ 77 kDa |
属性
形式 | Liquid |
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纯化 | Affinity purified. |
缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 50% Glycerol |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 |
Swiss-port # Q9UIS9 Human Methyl-CpG-binding domain protein 1 |
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基因名称 | MBD1 |
全名 | methyl-CpG binding domain protein 1 |
背景介绍 | The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011] |
生物功能 | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. [UniProt] |
细胞定位 | Nucleus. Nucleus matrix. Nucleus speckle. Chromosome. Note=Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. [UniProt] |
预测分子量 | 67 kDa |
翻译后修饰 | Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP. [UniProt] |
检测图片 (1) Click the Picture to Zoom In