ARG23263
anti-MAdCAM1 antibody [314G8]
anti-MAdCAM1 antibody [314G8] for ELISA,Flow cytometry,IHC-Frozen sections and Human
概述
| 产品描述 | Mouse Monoclonal antibody [314G8] recognizes MAdCAM1 Mouse anti Human MAdCAM-1 antibody, clone 314G8 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (Leung et al. 2004). |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, IHC-Fr |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 314G8 |
| 同位型 | IgG1 |
| 靶点名称 | MAdCAM1 |
| 抗原物种 | Human |
| 抗原 | Recombinant soluble MAdCAM-1-fc fusion protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Mucosal addressin cell adhesion molecule 1; hMAdCAM-1; MAdCAM-1; MACAM1 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13477 Human Mucosal addressin cell adhesion molecule 1 |
|---|---|
| 基因名称 | MADCAM1 |
| 全名 | mucosal vascular addressin cell adhesion molecule 1 |
| 背景介绍 | The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. [UniProt] |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. [UniProt] |
