ARG63125
anti-MAX antibody
anti-MAX antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes MAX |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_002373.3; NP_660087.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MAX |
| 抗原物种 | Human |
| 抗原 | C-EEPQSRKKLRMEAS |
| 偶联标记 | Un-conjugated |
| 別名 | Protein max; Myc-associated factor X; bHLHd4; Class D basic helix-loop-helix protein 4 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. [provided by RefSeq, Jul 2008] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 18 kDa |
| 翻译后修饰 | Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes. |
检测图片 (5) Click the Picture to Zoom In
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ARG63125 anti-MAX antibody WB image
Western Blot: Jurkat lysate (RIPA buffer, 1.4*10^5 cells per lane) stained with ARG63125 anti-MAX antibody at 0.1 µg/ml dilution.
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ARG63125 anti-MAX antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63125 anti-MAX antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63125 anti-MAX antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63125 anti-MAX antibody at 10 µg/ml dilution followed by AP-staining.
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ARG63125 anti-MAX antibody WB image
Western blot: 35 µg of Jurkat cell lysate (in RIPA buffer) stained with ARG63125 anti-MAX antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
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ARG63125 anti-MAX antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG63125 anti-MAX antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
