ARG40410
anti-MARK1 antibody
anti-MARK1 antibody for Western blot and Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MARK1 |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MARK1 |
| 抗原物种 | Human |
| 抗原 | Fusion protein of Human MARK1. |
| 偶联标记 | Un-conjugated |
| 別名 | PAR1 homolog c; Serine/threonine-protein kinase MARK1; EC 2.7.11.26; MARK; Par1c; Par-1c; EC 2.7.11.1; MAP/microtubule affinity-regulating kinase 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | WB: Mouse heart and brain tissue. | ||||
| 实际分子量 | ~ 90 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8VHJ5 Mouse Serine/threonine-protein kinase MARK1 |
|---|---|
| 基因名称 | MARK1 |
| 全名 | MAP/microtubule affinity-regulating kinase 1 |
| 生物功能 | Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). [UniProt] |
| 细胞定位 | Cell membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Note=Appears to localize to an intracellular network. [UniProt] |
| 预测分子量 | 89 kDa |
| 翻译后修饰 | Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity (By similarity). Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40410 anti-MARK1 antibody WB image
Western blot: 40 µg of Mouse heart and Mouse brain lysates stained with ARG40410 anti-MARK1 antibody at 1:500 dilution.
