ARG51772
anti-MARCKS phospho (Ser158) antibody
anti-MARCKS phospho (Ser158) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MARCKS phospho (Ser158) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MARCKS |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from Human MARCKS. |
| 偶联标记 | Un-conjugated |
| 別名 | MACS; 80K-L; Myristoylated alanine-rich C-kinase substrate; PKCSL; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; MARCKS; PRKCSL |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
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| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MARCKS |
| 全名 | myristoylated alanine-rich protein kinase C substrate |
| 背景介绍 | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. |
| 生物功能 | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 32 kDa |
| 翻译后修饰 | Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG51772 anti-MARCKS phospho (Ser158) antibody WB image
Western blot: Extracts from 3T3 cells stained with ARG51772 anti-MARCKS phospho (Ser158) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
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ARG51772 anti-MARCKS phospho (Ser158) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51772 anti-MARCKS phospho (Ser158) antibody.
