ARG52330
anti-MARCKS phospho (Ser152 / Ser156) antibody
anti-MARCKS phospho (Ser152 / Ser156) antibody for Western blot and Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MARCKS phospho (Ser152 / Ser156) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Chk, Xenopus laevis, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MARCKS |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | MACS; 80K-L; Myristoylated alanine-rich C-kinase substrate; PKCSL; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; MARCKS; PRKCSL |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by λ-phosphatase * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Marcks |
| 全名 | myristoylated alanine rich protein kinase C substrate |
| 背景介绍 | Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994). |
| 产品亮点 | Related Antibody Duos and Panels: ARG30216 Phospho MARCKS Antibody Duo for Rat (Total, pS152/156) Related products: MARCKS antibodies; MARCKS Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 32 kDa |
| 翻译后修饰 | Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG52330 anti-MARCKS phospho (Ser152 / Ser156) antibody WB image
Western blot: Rat brain lysate showing phospho-specific immunolabeling of the ~87k MARCKS protein stained with ARG52330 anti-MARCKS phospho (Ser152 / Ser156) antibody.
