ARG64709
anti-MARCH10 antibody
anti-MARCH10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes MARCH10 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Cow |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MARCH10 |
| 抗原物种 | Human |
| 抗原 | C-SRNYPQPRTEE |
| 偶联标记 | Un-conjugated |
| 別名 | Probable E3 ubiquitin-protein ligase MARCH10; EC 6.3.2.-; Membrane-associated RING finger protein 10; RNF190; MARCH-X; Membrane-associated RING-CH protein X; RING finger protein 190 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q5XIV2 Rat Probable E3 ubiquitin-protein ligase MARCH10 Swiss-port # Q8NA82 Human Probable E3 ubiquitin-protein ligase MARCH10 |
|---|---|
| 背景介绍 | MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 91 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG64709 anti-MARCH10 antibody WB image
Western Blot: Rat Testis lysate (35 µg protein in RIPA buffer) stained with ARG64709 anti-MARCH10 antibody at 0.3 µg/ml dilution.
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ARG64709 anti-MARCH10 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64709 anti-MARCH10 antibody at 5 µg/ml dilution followed by AP-staining.
