ARG43642
anti-MAPRE1 / EB1 antibody
anti-MAPRE1 / EB1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MAPRE1 / EB1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MAPRE1 / EB1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein fragment of Human MAPRE1 / EB1. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Microtubule-associated protein RP/EB family member 1 |
| 別名 | EB1; APC-binding protein EB1; End-binding protein 1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 30-35 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.01% Thimerosal and 50% Glycerol. |
| 抗菌剂 | 0.01% Thimerosal |
| 稳定剂 | 50% Glycerol |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MAPRE1 |
| 全名 | microtubule associated protein RP/EB family member 1 |
| 背景介绍 | The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:23001180, PubMed:28726242, PubMed:28814570, PubMed:34608293). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed:12388762, PubMed:34608293). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242). May play a role in cell migration (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm; Cytoskeleton; Golgi apparatus; Microtubule |
| 预测分子量 | 30 kDa |
| 翻译后修饰 | Acetylation; Phosphoprotein |
检测图片 (3) Click the Picture to Zoom In
-
ARG43642 anti-MAPRE1 / EB1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG43642 anti-MAPRE1 / EB1 antibody at 1:100 dilution, overnight at 4°C.
-
ARG43642 anti-MAPRE1 / EB1 antibody WB image
Western blot: Mouse lung and Mouse spleen tissue were stained with ARG43642 anti-MAPRE1 / EB1 antibody at 1:10000 dilution, overnight at 4°C.
-
ARG43642 anti-MAPRE1 / EB1 antibody WB image
Western blot: U-87MG and LO2 cell lysates were stained with ARG43642 anti-MAPRE1 / EB1 antibody at 1:10000 dilution, overnight at 4°C.
