ARG51783
anti-MAPKAPK2 / MK2 phospho (Thr334) antibody
anti-MAPKAPK2 / MK2 phospho (Thr334) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MAPKAPK2 / MK2 phospho (Thr334) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MAPKAPK2 / MK2 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of Threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK2 / MK2. |
| 偶联标记 | Un-conjugated |
| 別名 | MAPKAP-K2; MAPK-activated protein kinase 2; MK-2; MAPKAP kinase 2; MAP kinase-activated protein kinase 2; MK2; EC 2.7.11.1; MAPKAPK-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P49137 Human MAP kinase-activated protein kinase 2 Swiss-port # P49138 Mouse MAP kinase-activated protein kinase 2 |
|---|---|
| 基因名称 | MAPKAPK2 |
| 全名 | mitogen-activated protein kinase-activated protein kinase 2 |
| 背景介绍 | MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. |
| 生物功能 | Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | Sumoylation inhibits the protein kinase activity. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334. |
检测图片 (4) Click the Picture to Zoom In
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ARG51783 anti-MAPKAPK2 / MK2 phospho (Thr334) antibody WB image
Western blot: 25 µg of HeLa cells untreated or treated by UV. The blots were stained with ARG51783 anti-MAPKAPK2 / MK2 phospho (Thr334) antibody.
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ARG51783 anti-MAPKAPK2 / MK2 phospho (Thr334) antibody ICC/IF image
Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51783 anti-MAPKAPK2 / MK2 phospho (Thr334) antibody.
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ARG51783 anti MAPKAPK2 / MK2 phospho (Thr334) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51783 anti-MAPKAPK2 / MK2 phospho (Thr334) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51783 anti-MAPKAPK2 / MK2 phospho (Thr334) antibody WB image
Western blot: Extracts from HeLa cells untreated (lane 1) or treated with UV (lane 2) stained with ARG51783 anti-MAPKAPK2 / MK2 phospho (Thr334) antibody.
