ARG45905
anti-MAP4K4 / NIK antibody
anti-MAP4K4 / NIK antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MAP4K4 / NIK |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MAP4K4 / NIK |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to middle region of human MAP4K4 / NIK. |
| 偶联标记 | Un-conjugated |
| 別名 | MAP4K4; HGK; KIAA0687; NIK; Mitogen-activated protein kinase kinase kinase kinase 4; EC 2.7.11.1; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 160 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O95819 Human Mitogen-activated protein kinase kinase kinase kinase 4 Swiss-port # P97820 Mouse Mitogen-activated protein kinase kinase kinase kinase 4 |
|---|---|
| 基因名称 | MAP4K4 |
| 全名 | Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 |
| 背景介绍 | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 142 kDa |
| 翻译后修饰 | Acetylation; Phosphoprotein. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
-
ARG45905 anti-MAP4K4 / NIK antibody IHC-P image
Immunohistochemistry: Human cervical cancer stained with ARG45905 anti-MAP4K4 / NIK antibody at 2 μg/ml dilution.
-
ARG45905 anti-MAP4K4 / NIK antibody WB image
Western blot: Jurkat stained with ARG45905 anti-MAP4K4 / NIK antibody at 0.5 μg/ml dilution.
-
ARG45905 anti-MAP4K4 / NIK antibody FACS image
Flow Cytometry: Jurkat stained with ARG45905 anti-MAP4K4 / NIK antibody at 1 µg/10^6 cells dilution.
-
ARG45905 anti-MAP4K4 / NIK antibody WB image
Western blot: C6 stained with ARG45905 anti-MAP4K4 / NIK antibody at 0.5 μg/ml dilution.
-
ARG45905 anti-MAP4K4 / NIK antibody WB image
Western blot: NIH/3T3 stained with ARG45905 anti-MAP4K4 / NIK antibody at 0.5 μg/ml dilution.
