ARG57016
anti-MAFK antibody [2F7]
anti-MAFK antibody [2F7] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [2F7] recognizes MAFK |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2F7 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | MAFK |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-156 of Human MAFK. |
| 偶联标记 | Un-conjugated |
| 別名 | P18; NFE2U; Transcription factor MafK; Erythroid transcription factor NF-E2 p18 subunit |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MAFK |
| 全名 | v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog K |
| 背景介绍 | The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008] |
| 生物功能 | Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. [UniProt] |
| 预测分子量 | 18 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG57016 anti-MAFK antibody [2F7] WB image
Western blot: 30 µg of Jurkat cell lysate stained with ARG57016 anti-MAFK antibody [2F7] at 1:1000.
-
ARG57016 anti-MAFK antibody [2F7] WB image
Western blot: 20 ng of 1) Recombinant Human MAFK, 2) Recombinant Human MAFG, 3) Recombinant Human MAFF stained with ARG57016 anti-MAFK antibody [2F7] at 1:1000.
