ARG59420
anti-MAF1 antibody
anti-MAF1 antibody for Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MAF1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MAF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-256 of Human MAF1 (NP_115648.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Repressor of RNA polymerase III transcription MAF1 homolog |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | A549 and Mouse thymus | ||||
| 实际分子量 | 37 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9D0U6 Mouse Repressor of RNA polymerase III transcription MAF1 homolog Swiss-port # Q9H063 Human Repressor of RNA polymerase III transcription MAF1 homolog |
|---|---|
| 基因名称 | MAF1 |
| 全名 | MAF1 homolog, negative regulator of RNA polymerase III |
| 背景介绍 | This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq, Jul 2008] |
| 生物功能 | Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. [UniProt] |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Phosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function. Sumoylated with SUMO1 and SUMO2, mainly on Lys-35. Desumoylated by SENP1. SUMOylation promotes the ability of MAF1 to repress transcription and suppress colony formation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59420 anti-MAF1 antibody WB image
Western blot: 25 µg of A549 and Mouse thymus lysates stained with ARG59420 anti-MAF1 antibody at 1:1000 dilution.
