ARG45422
anti-MAD1 antibody
anti-MAD1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MAD1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MAD1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human MAD1. |
| 偶联标记 | Un-conjugated |
| 別名 | MAD1L1; Mitotic spindle assembly checkpoint protein MAD1; Tax-binding protein 181; MAD1-like protein 1; MAD1; Mitotic checkpoint MAD1 protein homolog; hMAD1; HsMAD1; TP53I9; PIG9; TXBP181; Mitotic arrest deficient 1-like protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 83 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9WTX8 Mouse Mitotic spindle assembly checkpoint protein MAD1 Swiss-port # Q9Y6D9 Human Mitotic spindle assembly checkpoint protein MAD1 |
|---|---|
| 基因名称 | MAD1L1 |
| 全名 | MAD1 mitotic arrest deficient-like 1 (yeast) |
| 背景介绍 | MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| 生物功能 | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. [UniProt]. [UniProt] |
| 细胞定位 | Chromosome; Cytoplasm; Nucleus; centromere; centrosome; cytoskeleton; kinetochore; microtubule organizing center; spindle. [UniProt]. [UniProt] |
| 预测分子量 | 83 kDa |
| 翻译后修饰 | Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. [UniProt]. [UniProt]. [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG45422 anti-MAD1 antibody IHC-P image
Immunohistochemistry: Human intestinal cancer stained with ARG45422 anti-MAD1 antibody at 1 μg/ml dilution.
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ARG45422 anti-MAD1 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG45422 anti-MAD1 antibody at 5 μg/ml dilution.
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ARG45422 anti-MAD1 antibody WB image
Western blot: THP-1 and Caco-2 stained with ARG45422 anti-MAD1 antibody at 0.5 μg/ml dilution.
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ARG45422 anti-MAD1 antibody FACS image
Flow Cytometry: A431 stained with ARG45422 anti-MAD1 antibody at 1 µg/10^6 cells dilution.
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ARG45422 anti-MAD1 antibody IHC-P image
Immunohistochemistry: Rat intestine stained with ARG45422 anti-MAD1 antibody at 1 μg/ml dilution.
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ARG45422 anti-MAD1 antibody WB image
Western blot: Rat lung stained with ARG45422 anti-MAD1 antibody at 0.5 μg/ml dilution.
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ARG45422 anti-MAD1 antibody IHC-P image
Immunohistochemistry: Mouse intestine stained with ARG45422 anti-MAD1 antibody at 1 μg/ml dilution.
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ARG45422 anti-MAD1 antibody WB image
Western blot: Mouse brain stained with ARG45422 anti-MAD1 antibody at 0.5 μg/ml dilution.
