ARG22678
anti-Ly-6B.2 antibody [7/4] (PE)
anti-Ly-6B.2 antibody [7/4] (PE) for Flow cytometry and Mouse
概述
| 产品描述 | PE-conjugated Rat Monoclonal antibody [7/4] recognizes Ly-6B.2 This antibody recognizes Ly-6B.2 antigen. Ly-6B.2 is a ~25-30kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15kDa (Rosas et al.2010). In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA. T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982). Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010). |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | 7/4 |
| 同位型 | IgG2a |
| 靶点名称 | Ly-6B.2 |
| 抗原物种 | Mouse |
| 抗原 | Cultured bone marrow cells |
| 偶联标记 | PE |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (1) Click the Picture to Zoom In
-
ARG22678 anti-Ly-6B.2 antibody [7/4] (PE) FACS image
Flow Cytometry: C57Bl/6 Mouse peripheral granulocytes stained with ARG22678 anti-Ly-6B.2 antibody [7/4] (PE).
