ARG10736

anti-Laminin 111 antibody

Datasheet

参考文献2

订购信息

包装

价格 (USD)

50 μl

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes Laminin 111
反应物种	Hu, Ms, Rat
应用	ICC/IF, IHC-Fr, WB
特异性	ARG10736 Laminin-111 antibody recognizes 3 laminin isotypes: α1 (440kDa), β1 (220kD) and γ1 (220kDa).
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	Laminin 111
抗原物种	Mouse
抗原	Laminin isolated from Mouse EHS cells.
偶联标记	Un-conjugated
別名	LAMA; PTBHS; S-LAM-alpha; Laminin subunit alpha-1; laminin, beta 1; laminin, gamma 1

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:1000 - 1:5000
IHC-Fr	1:1000 - 1:5000
WB	1:1000 - 1:5000

应用说明

Note: Based on the reference PMID: 35453580 This antibody may also suitable for IHC-P application in human samples.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification.
缓冲液	PBS and 50% Glycerol.
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 16772 Mouse LAMA1 GeneID: 284217 Human LAMA1 Swiss-port # P19137 Mouse Laminin subunit alpha-1 Swiss-port # P25391 Human Laminin subunit alpha-1
基因名称	Lama1
全名	laminin, alpha 1; laminin, beta 1; laminin, gamma 1
背景介绍	This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]
生物功能	Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. [UniProt]
细胞定位	Secreted › extracellular space › extracellular matrix › basement membrane Note: Major component.
预测分子量	337 kDa
翻译后修饰	Tyrosine phosphorylated by PKDCC/VLK.

检测图片 (3) Click the Picture to Zoom In

ARG10736 anti-Laminin 111 antibody IHC-Fr image

Immunohistochemistry: Frozen section of Rat brain stem stained with ARG10736 anti-Laminin 111 antibody (red) at 1:1000 dilution and costained with ARG52344 anti-Myelin Basic Protein antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)

The Laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.
ARG10736 anti-Laminin 111 antibody IHC-Fr image

Immunohistochemistry: Frozen sections of Mouse cortex stained with ARG10736 anti-Laminin 111 antibody (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.
ARG10736 anti-Laminin 111 antibody WB image

Western blot: 1) Rat heart cells lysates, and 2) 0.2 µg of purified laminin-111 protein from Mouse EHS sarcoma was stained with ARG10736 anti-Laminin 111 antibody. This antibody recognizes 3 laminin isotypes: α1 (440 kDa), β1 (220kD) and γ1 (220 kDa). Also recognized a laminin binding protein at 120 kDa in both Rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this cross reactivity is irrelevant.