ARG10836
anti-Lamin B Receptor antibody [BB2SS3F3]
anti-Lamin B Receptor antibody [BB2SS3F3] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [BB2SS3F3] recognizes Lamin B Receptor |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BB2SS3F3 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Lamin B Receptor |
| 抗原物种 | Human |
| 抗原 | GST fused protein around aa. 1-211 (N-terminus) of Human Lamin B Receptor. |
| 偶联标记 | Un-conjugated |
| 別名 | PHA; LMN2R; TDRD18; DHCR14B; Integral nuclear envelope inner membrane protein; Lamin-B receptor |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa, C2C12 and NIE-116 cells. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LBR |
| 全名 | lamin B receptor |
| 背景介绍 | The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Anchors the lamina and the heterochromatin to the inner nuclear membrane. [UniProt] |
| 预测分子量 | 71 kDa |
| 翻译后修饰 | Phosphorylated by CDK1 in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated by SRPK1. In late anaphase LBR is dephosphorylated, probably by PP1 and/or PP2A, allowing reassociation with chromatin. |
检测图片 (4) Click the Picture to Zoom In
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ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3] ICC/IF image
Immunofluorescence: Confocal images of HeLa and Mouse NIE-115 cell lines stained with ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3]. Left: Primary antibodies (green). Middle: Hoechst (blue). Right: Merge.
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ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3] IHC-Fr image
Immunohistochemistry: Small intestine from adult C57BL/6 mice was transferred to OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, permeabilized and stained with ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3].
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ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3] WB image
Western blot: Whole cell lysate from HeLa, C2C12, immortalized Mouse adult fibroblasts (MAFs) and NIE-115 cell lysates stained with ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3].
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ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3] ICC/IF image
Immunofluorescence: 3T3 expressing mCherry-LBR (upper panel), Wild-type immortalized mouse adult fibroblast (MAFs LBR+/+) (middle panel) and LBR-null MEFs (MAFs LBR-/-) (lower panel) were methanol fixed and stained with ARG10836 anti-Lamin B Receptor antibody [BB2SS3F3]. The nuclei were counter-stained with Hoechst. Rightmost: Merged picture.
