ARG65475
anti-LST1 antibody [LST1/02]
anti-LST1 antibody [LST1/02] for ICC/IF,Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Neuroscience antibody
概述
| 产品描述 | Mouse Monoclonal antibody [LST1/02] recognizes LST1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IP, WB |
| 特异性 | The clone LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule._x000D_ |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | LST1/02 |
| 同位型 | IgG1 |
| 靶点名称 | LST1 |
| 抗原物种 | Human |
| 抗原 | KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1_x000D_ |
| 偶联标记 | Un-conjugated |
| 別名 | Protein B144; B144; D6S49E; LST-1; Leukocyte-specific transcript 1 protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: It migrates on SDS-PAGE gels as ~ 25-28 kDa molecule. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IP: Raji |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00453 Human Leukocyte-specific transcript 1 protein |
|---|---|
| 基因名称 | LST1 |
| 全名 | leukocyte specific transcript 1 |
| 背景介绍 | LST1 (leukocyte-specific transcript, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A – LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec._x000D_ |
| 生物功能 | Possible role in modulating immune responses. Induces morphological changes including production of filopodia and microspikes when overexpressed in a variety of cell types and may be involved in dendritic cell maturation. Isoform 1 and isoform 2 have an inhibitory effect on lymphocyte proliferation. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody; Neuroscience antibody |
| 预测分子量 | 11 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG65475 anti-LST1 antibody [LST1/02] WB image
Western blot: U937 cell lysate stained with antibodies.
Lane 1: immunostaining with Isotype mouse IgG1 control; Lane 2-3: immunostaining with ARG65475 anti-LST1 antibody [LST1/02], two independent batches. Note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
