ARG43132
anti-LRTOMT antibody
anti-LRTOMT antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LRTOMT |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LRTOMT |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human LRTOMT. (RLLTVERDPRTAAVAEKLIRLAGFDEHMVEL) |
| 偶联标记 | Un-conjugated |
| 別名 | Protein LRTOMT1; LRRC51; CFAP111; DFNB63; Leucine-rich repeat-containing protein 51 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 27 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # B6CZ62 Rat Transmembrane O-methyltransferase Swiss-port # Q96E66 Human Leucine-rich repeat-containing protein 51 |
|---|---|
| 基因名称 | LRTOMT |
| 全名 | leucine rich transmembrane and O-methyltransferase domain containing |
| 背景介绍 | This gene has evolved in primates as a fusion of two ancestral neighboring genes, Lrrc51 and Tomt, which exist as two independent genes in rodents. The fusion gene contains some shared exons, but encodes structurally unrelated proteins, LRTOMT1 and LRTOMT2. Those variants that utilize the more centromere-proximal 3' terminal exon (short transcript form) encode LRTOMT1, while those variants that use a more centromere-distal 3' terminal exon (long transcript form) encode the LRTOMT2 protein. There is a small region within one of the exons of this gene that contains overlapping alternate reading frames for both LRTOMT1 and LRTOMT2. LRTOMT1 shares similarity with the protein encoded by mouse Lrrc51, while LRTOMT2 shares similarity with the protein encoded by mouse Tomt. Alternative splicing results in multiple transcript variants, encoding different isoforms of both LRTOMT1 and LRTOMT2. The LRTOMT1 protein is a leucine-rich repeat-containing protein, while the LRTOMT2 protein is a catechol-O-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to a hydroxyl group of catechols and is essential for auditory and vestibular function. Mutations in this gene have been associated with nonsyndromic deafness. [provided by RefSeq, Nov 2017] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 22 kDa |
检测图片 (7) Click the Picture to Zoom In
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ARG43132 anti-LRTOMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43132 anti-LRTOMT antibody WB image
Western blot: 50 µg of sample under reducing conditions. Human placenta, MCF-7, HeLa, Caco-2, K562, U2OS and THP-1 whole cell lysates stained with ARG43132 anti-LRTOMT antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43132 anti-LRTOMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43132 anti-LRTOMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43132 anti-LRTOMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43132 anti-LRTOMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43132 anti-LRTOMT antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain, Rat ovary, Rat heart, Rat lung, Mouse brain and Mouse lung lysates stained with ARG43132 anti-LRTOMT antibody at 0.5 µg/ml dilution, overnight at 4°C.
