ARG66441
anti-LRAT antibody
anti-LRAT antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LRAT |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LRAT |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 80-160 of Human LRAT. |
| 偶联标记 | Un-conjugated |
| 別名 | Phosphatidylcholine--retinol O-acyltransferase; Lecithin retinol acyltransferase; LCA14; EC 2.3.1.135 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LRAT |
| 全名 | lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) |
| 背景介绍 | The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
| 生物功能 | Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Single-pass membrane protein. Rough endoplasmic reticulum. Endosome, multivesicular body. Cytoplasm, perinuclear region. Note=Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells (By similarity). [UniProt] |
| 预测分子量 | 26 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG66441 anti-LRAT antibody ICC/IF image
Immunofluorescence: HUVEC cells stained with ARG66441 anti-LRAT antibody. The picture on the right is blocked with the synthetic peptide.
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ARG66441 anti-LRAT antibody WB image
Western blot: COLO205 cell lysate stained with ARG66441 anti-LRAT antibody at 1:500 dilution.
