ARG40404
anti-LMAN1 antibody
anti-LMAN1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LMAN1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LMAN1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human LMAN1. |
| 偶联标记 | Un-conjugated |
| 別名 | F5F8D; ERGIC-53; ER-Golgi intermediate compartment 53 kDa protein; FMFD1; Protein ERGIC-53; MR60; gp58; ERGIC53; Lectin mannose-binding 1; Intracellular mannose-specific lectin MR60; MCFD1; Gp58 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa | ||||||||
| 实际分子量 | ~ 58 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LMAN1 |
| 全名 | lectin, mannose-binding, 1 |
| 背景介绍 | The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015] |
| 生物功能 | Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. [UniProt] |
| 细胞定位 | Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | The N-terminal may be partly blocked. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40404 anti-LMAN1 antibody ICC/IF image
Immunofluorescence: JAR cells were fixed with 4% Polyoxymethylene and permeabilized with 0.1% Triton X-100. Cells were stained with ARG40404 anti-LMAN1 antibody (green) at 1:100 dilution. Nuclear staining (blue).
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ARG40404 anti-LMAN1 antibody WB image
Western blot: HeLa cell lysate stained with ARG40404 anti-LMAN1 antibody.
