ARG55700
anti-LIMK2 antibody
anti-LIMK2 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LIMK2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Bov |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LIMK2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 570-599 (C-terminus) of Human LIMK2. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.7.11.1; LIMK-2; LIM domain kinase 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Jurkat |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LIMK2 |
| 全名 | LIM domain kinase 2 |
| 背景介绍 | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. [UniProt] |
| 细胞定位 | Isoform LIMK2a: Cytoplasm. Nucleus. Note=Isoform LIMK2a is distributed in the cytoplasm and the nucleus |
| 预测分子量 | 72 kDa |
| 翻译后修饰 | Phosphorylated on serine and/or threonine residues by ROCK1. |
检测图片 (1) Click the Picture to Zoom In
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ARG55700 anti-LIMK2 antibody WB image
Western blot: 35 µg of Jurkat cell lysate stained with ARG55700 anti-LIMK2 antibody at 1:1000 dilution.
