ARG63581
anti-LIME1 antibody
anti-LIME1 antibody for Western blot and Mouse
Immune System antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes LIME1 |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Rat |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LIME1 |
| 抗原物种 | Mouse |
| 抗原 | C-ENVYESIKEMGL |
| 偶联标记 | Un-conjugated |
| 別名 | Lck-interacting molecule; Lck-interacting membrane protein; Lck-interacting transmembrane adapter 1; dJ583P15.4; LIME |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9EQR5 Mouse Lck-interacting transmembrane adapter 1 |
|---|---|
| 基因名称 | Lime1 |
| 全名 | Lck interacting transmembrane adaptor 1 |
| 背景介绍 | This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
| 生物功能 | Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. [UniProt] |
| 研究领域 | Immune System antibody |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | Palmitoylation of Cys-28 and Cys-31 is required for raft targeting. Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins. |
检测图片 (1) Click the Picture to Zoom In
-
ARG63581 anti-LIME1 antibody WB image
Western blot: Mouse Heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63581 anti-LIME1 antibody at 1 µg/ml dilution.
