概述

产品描述	Rabbit Polyclonal antibody recognizes LIG4
反应物种	Hu, Ms, Rat
应用	IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	LIG4
抗原物种	Human
抗原	Recombinant protein of Human LIG4.
偶联标记	Un-conjugated
別名	DNA ligase 4; DNA ligase IV; LIG4S; EC 6.5.1.1; Polydeoxyribonucleotide synthase [ATP] 4

应用说明

应用建议	应用 推荐稀释比 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	Jurkat

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 319583 Mouse LIG4 GeneID: 3981 Human LIG4 Swiss-port # P49917 Human DNA ligase 4 Swiss-port # Q8BTF7 Mouse DNA ligase 4
基因名称	LIG4
全名	ligase IV, DNA, ATP-dependent
背景介绍	The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
生物功能	Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. [UniProt]
研究领域	Gene Regulation antibody
预测分子量	104 kDa

检测图片 (1) Click the Picture to Zoom In