ARG42973
anti-LHX1 / LIM1 antibody
anti-LHX1 / LIM1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LHX1 / LIM1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LHX1 / LIM1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to M1-S317 of Human LHX1 / LIM1. |
| 偶联标记 | Un-conjugated |
| 別名 | LIM/homeobox protein Lhx1; Homeobox protein Lim-1; hLim-1; LIM1; LIM-1; LIM homeobox protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 44 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LHX1 |
| 全名 | LIM homeobox 1 |
| 背景介绍 | This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq, Dec 2010] |
| 生物功能 | Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 45 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG42973 anti-LHX1 / LIM1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42973 anti-LHX1 / LIM1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42973 anti-LHX1 / LIM1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Mouse brain lysate stained with ARG42973 anti-LHX1 / LIM1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42973 anti-LHX1 / LIM1 antibody FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum and then stained with ARG42973 anti-LHX1 / LIM1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
