ARG40335
anti-LCP1 / Plastin L antibody
anti-LCP1 / Plastin L antibody for Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LCP1 / Plastin L |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LCP1 / Plastin L |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-270 of Human LCP1 (NP_002289.2). |
| 偶联标记 | Un-conjugated |
| 別名 | LC64P; L-PLASTIN; CP64; HEL-S-37; LCP-1; LPL; L-plastin; Lymphocyte cytosolic protein 1; Plastin-2; PLS2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse spleen and DU145 | ||||||
| 实际分子量 | 67 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LCP1 |
| 全名 | lymphocyte cytosolic protein 1 (L-plastin) |
| 背景介绍 | Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008] |
| 生物功能 | Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. Cell junction. Cell projection. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Relocalizes to the immunological synapse between peripheral blood T-lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Associated with the actin cytoskeleton at membrane ruffles. Relocalizes to actin-rich cell projections upon serine phosphorylation (PubMed:16636079). [UniProt] |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40335 anti-LCP1 / Plastin L antibody WB image
Western blot: 25 µg of DU145 cell lysate stained with ARG40335 anti-LCP1 / Plastin L antibody at 1:1000 dilution.
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ARG40335 anti-LCP1 / Plastin L antibody IP image
Immunoprecipitation: 150 µg extracts of Jurkat cells immunoprecipitated and stained with ARG40335 anti-LCP1 / Plastin L antibody at 1:1000 dilution.
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ARG40335 anti-LCP1 / Plastin L antibody WB image
Western blot: 25 µg of Mouse spleen lysate stained with ARG40335 anti-LCP1 / Plastin L antibody at 1:1000 dilution.
