ARG66834
anti-LATS1 phospho (Thr1079) + LATS2 phospho (Thr1041) antibody
anti-LATS1 phospho (Thr1079) + LATS2 phospho (Thr1041) antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LATS1 phospho (Thr1079) + LATS2 phospho (Thr1041) |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | ELISA, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LATS1 + LATS2 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Thr1079 of Human LATS1. Phosphospecific peptide around Thr1041 of Human LATS2. |
| 偶联标记 | Un-conjugated |
| 別名 | LATS1: wts; Serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; h-warts; EC 2.7.11.1; Large tumor suppressor homolog 1 LATS2: KPM |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | LATS1; LATS2 |
|---|---|
| 全名 | large tumor suppressor kinase 1 large tumor suppressor kinase 2 |
| 背景介绍 | LATS1: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017] LATS2: This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008] |
| 生物功能 | Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. [UniProt] |
| 预测分子量 | LATS1: 127 kDa LATS2: 120 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG66834 anti-LATS1 phospho (Thr1079) + LATS2 phospho (Thr1041) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66834 anti-LATS1 phospho (Thr1079) + LATS2 phospho (Thr1041) antibody. The picture on the right is blocked with the phospho peptide.
