ARG63056
anti-LAT antibody [LAT-01]
anti-LAT antibody [LAT-01] for Flow cytometry,Immunoprecipitation,Western blot and Human
Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [LAT-01] recognizes LAT |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms |
| 应用 | FACS, IP, WB |
| 特异性 | The clone LAT-01 reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | LAT-01 |
| 同位型 | IgG1 |
| 靶点名称 | LAT |
| 抗原物种 | Human |
| 抗原 | Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT. |
| 偶联标记 | Un-conjugated |
| 別名 | Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; LAT1; p36-38 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IP: Tyrosine-phosphorylated form of LAT is not optimally recognized. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O43561 Human Linker for activation of T-cells family member 1 |
|---|---|
| 基因名称 | LAT |
| 全名 | linker for activation of T cells |
| 背景介绍 | LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation. |
| 生物功能 | Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. [UniProt] |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes. Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. |
检测图片 (1) Click the Picture to Zoom In
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ARG63056 anti-LAT antibody [LAT-01] FACS image
Flow Cytometry: Peripheral blood lymphocytes stained with ARG63056 anti-LAT antibody [LAT-01]. (surface markers were stained prior to intracellular staining of human LAT).
Panel A: gate on CD4+ cells, Panel B: gate on CD8+ cells.
