ARG65221
anti-LARGE1 antibody
anti-LARGE1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes LARGE1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Dog |
| 应用 | WB |
| 特异性 | Reported variants represent identical protein: NP_004728.1, NP_598397.1 |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LARGE1 |
| 抗原物种 | Human |
| 抗原 | C-SEADVNSENLQKQ |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.4.1.-; MDC1D; EC 2.4.-.-; Acetylglucosaminyltransferase-like 1A; MDDGA6; MDDGB6; Glycosyltransferase-like protein LARGE1; EC 2.4.2.- |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O95461 Human Glycosyltransferase-like protein LARGE1 |
|---|---|
| 基因名称 | LARGE1 |
| 全名 | LARGE xylosyl- and glucuronyltransferase 1 |
| 背景介绍 | This gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. Mutations in this gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008] |
| 生物功能 | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 88 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG65221 anti-LARGE1 antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG65221 anti-LARGE1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
