ARG62530
anti-L1CAM antibody [UJ127]
anti-L1CAM antibody [UJ127] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [UJ127] recognizes L1CAM |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | UJ127 |
| 同位型 | IgG1 |
| 靶点名称 | L1CAM |
| 抗原物种 | Human |
| 抗原 | Homogenous suspension of 16 week human fetal brain |
| 偶联标记 | Un-conjugated |
| 別名 | SPG1; CD171; NCAM-L1; MASA; MIC5; S10; CAML1; HSAS1; N-CAM-L1; CD antigen CD171; N-CAML1; HSAS; Neural cell adhesion molecule L1 |
应用说明
| 应用说明 | IHC: 1 - 2 µg/ml IP: 2 µg/mg of lysate WB: 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| 阳性对照 | IMR-5 cells |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | L1CAM |
| 全名 | L1 cell adhesion molecule |
| 背景介绍 | The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013] |
| 生物功能 | Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. [UniProt] |
| 细胞定位 | Cell membrane |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 140 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG62530 anti-L1CAM antibody [UJ127] WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG62530 anti-L1CAM antibody [UJ127] at 1:500 dilution.
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anti-L1CAM antibody [UJ127]
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
克隆号文献
EphB regulates L1 phosphorylation during retinocollicular mapping.
IP /
The dimensions and characteristics of the subepidermal nerve plexus in human skin--terminal Schwann cells constitute a substantial cell population within the superficial dermis.
ICC/IF / Human
Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.
WB / Human
Two alcohol binding residues interact across a domain interface of the L1 neural cell adhesion molecule and regulate cell adhesion.
FACS / Human
Modification of the L1-CAM carboxy-terminus in pancreatic adenocarcinoma cells.
IHC / Human
