ARG62529
anti-Ku 70 antibody [N3H10]
anti-Ku 70 antibody [N3H10] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Monkey
Cancer antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [N3H10] recognizes Ku 70 |
|---|---|
| 反应物种 | Hu, Ms, Mk |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | N3H10 |
| 同位型 | IgG2b |
| 靶点名称 | Ku 70 |
| 抗原物种 | Human |
| 抗原 | Human placental extract designated as PSE1-PL. |
| 抗原表位 | Amino acids 506 - 541 |
| 偶联标记 | Un-conjugated |
| 別名 | DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70 |
应用说明
| 应用说明 | WB: 0.25 - 0.5 µg/ml IP: 2µg/mg of protein lysate IF: 1 µg/ml FACS: 1µg for 106 cells IHC: 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purified |
| 缓冲液 | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P12956 Human X-ray repair cross-complementing protein 6 Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6 |
|---|---|
| 基因名称 | XRCC6 |
| 全名 | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| 背景介绍 | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008] |
| 生物功能 | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Cancer antibody; Gene Regulation antibody |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. |
克隆号文献
ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency.
P21-PARP-1 pathway is involved in cigarette smoke-induced lung DNA damage and cellular senescence.
WB / Mouse
A mechanism for the inhibition of DNA-PK-mediated DNA sensing by a virus.
WB / Human
A noncatalytic function of the ligation complex during nonhomologous end joining.
WB / Human
Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells.
ChIP / Human
Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11.
ICC/IF / Human
Treatment of PC12 cells with nerve growth factor induces proteasomal degradation of T-cadherin that requires tyrosine phosphorylation of its cadherin domain.
WB / Rat
Expression and chromosome location of hamster Ku70 and Ku80.
WB / Hamster
A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera.
IP / Human
Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.
FACS / Human
