ARG57831

anti-Ku 70 antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

100 μl

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes Ku 70
反应物种	Hu, Ms, Rat
应用	ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	Ku 70
抗原物种	Human
抗原	Synthetic peptide of Human Ku70.
偶联标记	Un-conjugated
別名	DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:50 - 1:100
IHC-P	1:50 - 1:100
WB	1:500 - 1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

SW480

实际分子量

~ 70 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 14375 Mouse XRCC6 GeneID: 2547 Human XRCC6 Swiss-port # P12956 Human X-ray repair cross-complementing protein 6 Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6
基因名称	XRCC6
全名	X-ray repair complementing defective repair in Chinese hamster cells 6
背景介绍	The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
生物功能	Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]
细胞定位	Chromosome, Nucleus. [UniProt]
预测分子量	Isoform 1: 70 kDa Isoform 2: 65 kDa
翻译后修饰	Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. [UniProt]

检测图片 (3) Click the Picture to Zoom In

ARG57831 anti-Ku 70 antibody ICC/IF image

Immunofluorescence: HeLa cells stained with ARG57831 anti-Ku 70 antibody at 1:100 dilution.
ARG57831 anti-Ku 70 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human brain astrocytoma stained with ARG57831 anti-Ku 70 antibody at 1:100 dilution.
ARG57831 anti-Ku 70 antibody WB image

Western blot: 25 µg of SW480 cell lysate stained with ARG57831 anti-Ku 70 antibody at 1:1000 dilution.