ARG55041
anti-Ki-67 antibody [Ki-67] (PE)
anti-Ki-67 antibody [Ki-67] (PE) for Flow cytometry and Human,Cow
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [Ki-67] recognizes Ki-67 |
|---|---|
| 反应物种 | Hu, Cow |
| 应用 | FACS |
| 特异性 | The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | Ki-67 |
| 同位型 | IgG1 |
| 靶点名称 | Ki-67 |
| 抗原物种 | Human |
| 抗原 | Nuclei of the Hodgkin lymphoma cell line L428. |
| 偶联标记 | PE |
| 別名 | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 15 mM Sodium azide. |
| 抗菌剂 | 15 mM Sodium azide |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MKI67 |
| 全名 | marker of proliferation Ki-67 |
| 背景介绍 | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
| 生物功能 | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
| 细胞定位 | Chromosome. Nucleus, nucleolus. Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface. Associates with satellite DNA in G1 phase. Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes. Predominantly localized in the G1 phase in the perinucleolar region. [UniProt] |
| 预测分子量 | 359 kDa |
| 翻译后修饰 | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG55041 anti-Ki-67 antibody [Ki-67] (PE) FACS image
Flow Cytometry: Human peripheral blood mononuclear cells stimulated with PHA. Surface staining of ARG53800 anti-CD25 antibody [MEM-181] (APC) was followed by permeabilization and nuclear staining of ARG55041 anti-Ki-67 antibody [Ki-67] (PE).
