ARG55274
anti-KPNA4 / IPOA3 antibody
anti-KPNA4 / IPOA3 antibody for ICC/IF,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes KPNA4 / IPOA3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KPNA4 / IPOA3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (15 aa) within aa. 30-80 of Human KPNA4. |
| 偶联标记 | Un-conjugated |
| 別名 | SRP3; Importin subunit alpha-3; Qip1; Karyopherin subunit alpha-4; IPOA3; QIP1; Importin alpha Q1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human Liver Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KPNA4 |
| 全名 | karyopherin alpha 4 (importin alpha 3) |
| 背景介绍 | The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008] |
| 生物功能 | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 58 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG55274 anti-KPNA4 / IPOA3 antibody WB image
Western blot: HeLa cell lysate stained with ARG55274 anti-KPNA4 / IPOA3 antibody at (A) 0.5 and (B) 1 µg/ml dilution.
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ARG55274 anti-KPNA4 / IPOA3 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55274 anti-KPNA4 / IPOA3 antibody at 20 µg/ml dilution.
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ARG55274 anti-KPNA4 / IPOA3 antibody ICC/IF image
Immunocytochemistry: HeLa cells stained with ARG55274 anti-KPNA4 / IPOA3 antibody at 2.5 µg/ml dilution.
