ARG63401
anti-KPNA2 / IPOA1 antibody
anti-KPNA2 / IPOA1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes KPNA2 / IPOA1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KPNA2 / IPOA1 |
| 抗原物种 | Human |
| 抗原 | C-QVQDGAPGTFNF |
| 偶联标记 | Un-conjugated |
| 別名 | Karyopherin subunit alpha-2; SRP1alpha; Importin subunit alpha-1; QIP2; RCH1; IPOA1; SRP1-alpha; RAG cohort protein 1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008] |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 58 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG63401 anti-KPNA2 / IPOA1 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG63401 anti-KPNA2 / IPOA1 antibody at 1:1000 dilution.
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ARG63401 anti-KPNA2 / IPOA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63401 anti-KPNA2 / IPOA1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63401 anti-KPNA2 / IPOA1 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG63401 anti-KPNA2 / IPOA1 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
客户反馈
Excellent
anti-KPNA2 / IPOA1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
