ARG54749
anti-KMT3A / SETD2 antibody
anti-KMT3A / SETD2 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes KMT3A / SETD2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KMT3A / SETD2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 21-50 (N-terminus) of Human KMT3A / SETD2 (NP_054878.5). |
| 偶联标记 | Un-conjugated |
| 別名 | HIF-1; SET domain-containing protein 2; SET2; Huntingtin-interacting protein B; Huntingtin yeast partner B; KMT3A; Huntingtin-interacting protein 1; hSET2; EC 2.1.1.43; HBP231; Lysine N-methyltransferase 3A; HIP-1; p231HBP; HSPC069; Histone-lysine N-methyltransferase SETD2; HYPB |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BYW2 Human Histone-lysine N-methyltransferase SETD2 |
|---|---|
| 基因名称 | SETD2 |
| 全名 | SET domain containing 2 |
| 背景介绍 | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008] |
| 生物功能 | Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. [UniProt] |
| 细胞定位 | Nucleus. Chromosome |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 288 kDa |
| 翻译后修饰 | May be automethylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG54749 anti-KMT3A / SETD2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma tissue stained with ARG54749 anti-KMT3A / SETD2 antibody.
