ARG54751
anti-KMT2E / MLL5 antibody
anti-KMT2E / MLL5 antibody for Western blot and Human
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes KMT2E / MLL5 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KMT2E / MLL5 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 93-120 (N-terminus) of Human MLL5 (NP_061152.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Lysine N-methyltransferase 2E; NKp44L; EC 2.1.1.43; MLL5; Histone-lysine N-methyltransferase 2E; HDCMC04P; Myeloid/lymphoid or mixed-lineage leukemia protein 5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HL-60 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8IZD2 Human Histone-lysine N-methyltransferase 2E |
|---|---|
| 基因名称 | KMT2E |
| 全名 | lysine (K)-specific methyltransferase 2E |
| 背景介绍 | This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic-acid-induced granulopoiesis by acting as a coactivator of RAR-alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Isoform NKp44L: Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity. [UniProt] |
| 细胞定位 | Nucleus speckle. Note=Absent from the nucleolus |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 205 kDa |
| 翻译后修饰 | O-glycosylation at Thr-440 in the SET domain by OGT is essential for the histone methyltransferase and the coactivator activity toward RARA in granulopoiesis. The absence of Thr-440 glycosylation in assays done in vitro may explain why some authors did not detected any histone methyltransferase activity for this protein. |
检测图片 (1) Click the Picture to Zoom In
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ARG54751 anti-KMT2E / MLL5 antibody WB image
Western blot: 35 µg of HL-60 cell lysate stained with ARG54751 anti-KMT2E / MLL5 antibody.
