ARG66465
anti-KLK10 / Kallikrein 10 antibody
anti-KLK10 / Kallikrein 10 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes KLK10 / Kallikrein 10 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KLK10 / Kallikrein 10 |
| 抗原物种 | Human |
| 抗原 | Recombinant full length protein of Human KLK10. |
| 偶联标记 | Un-conjugated |
| 別名 | Normal epithelial cell-specific 1; Protease serine-like 1; Kallikrein-10; PRSSL1; EC 3.4.21.-; NES1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KLK10 |
| 全名 | kallikrein-related peptidase 10 |
| 背景介绍 | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| 生物功能 | Has a tumor-suppressor role for NES1 in breast and prostate cancer. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 30 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG66465 anti-KLK10 / Kallikrein 10 antibody ICC/IF image
Immunofluorescence: Formalin-fixed A549 cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66465 anti-KLK10 / Kallikrein 10 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber.
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ARG66465 anti-KLK10 / Kallikrein 10 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver cancer. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then incubated with ARG66465 anti-KLK10 / Kallikrein 10 antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG66465 anti-KLK10 / Kallikrein 10 antibody WB image
Western blot: Mouse brain, Mouse heart and Rat liver lysates stained with ARG66465 anti-KLK10 / Kallikrein 10 antibody.
