ARG63222
anti-KLF8 / BKLF3 antibody
anti-KLF8 / BKLF3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes KLF8 / BKLF3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Pig |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KLF8 / BKLF3 |
| 抗原物种 | Human |
| 抗原 | VDMDKLINNLEVQ-C |
| 偶联标记 | Un-conjugated |
| 別名 | Krueppel-like factor 8; BKLF3; Basic krueppel-like factor 3; ZNF741; Zinc finger protein 741 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location. |
检测图片 (2) Click the Picture to Zoom In
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ARG63222 anti-KLF8 / BKLF3 antibody WB image
Western Blot: Human Kidney lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63222 anti-KLF8 / BKLF3 antibody at 2 µg/ml dilution.
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ARG63222 anti-KLF8 / BKLF3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63222 anti-KLF8 / BKLF3 antibody at 3.75 µg/ml dilution followed by AP-staining.
