ARG63587
anti-KLF3 / BKLF antibody
anti-KLF3 / BKLF antibody for Flow cytometry,ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes KLF3 / BKLF |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KLF3 / BKLF |
| 抗原物种 | Human |
| 抗原 | LMFDPVPVKQEAMD-C |
| 偶联标记 | Un-conjugated |
| 別名 | CACCC-box-binding protein BKLF; Krueppel-like factor 3; BKLF; Basic krueppel-like factor; TEF-2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KLF3 |
| 全名 | Kruppel-like factor 3 (basic) |
| 生物功能 | Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis (By similarity). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site (By similarity). |
检测图片 (4) Click the Picture to Zoom In
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ARG63587 anti-KLF3 / BKLF antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63587 anti-KLF3 / BKLF antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63587 anti-KLF3 / BKLF antibody WB image
Western blot: 35 µg of Human heart lysate (in RIPA buffer) stained with ARG63587 anti-KLF3 / BKLF antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63587 anti-KLF3 / BKLF antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG63587 anti-KLF3 / BKLF antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63587 anti-KLF3 / BKLF antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A549 cells permeabilized with 0.15% Triton. Cells were stained with ARG63587 anti-KLF3 / BKLF antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
