ARG63212
anti-KLF15 antibody
anti-KLF15 antibody for Flow cytometry,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes KLF15 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Pig |
| 应用 | FACS, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KLF15 |
| 抗原物种 | Human |
| 抗原 | VDHLLPVDENFSS-C |
| 偶联标记 | Un-conjugated |
| 別名 | Krueppel-like factor 15; Kidney-enriched krueppel-like factor; KKLF |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KLF15 |
| 全名 | Kruppel-like factor 15 |
| 生物功能 | Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CTGF expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 44 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG63212 anti-KLF15 antibody WB image
Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG63212 anti-KLF15 antibody at 0.5 µg/ml dilution.
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ARG63212 anti-KLF15 antibody WB image
Western blot: 35 µg of HepG2 nucleus (A) and HeLa (B) cell lysates (in RIPA buffer) stained with ARG63212 anti-KLF15 antibody at 1 µg/ml (A) and 0.5 µg/ml (B) dilutions and incubated at RT for 1 hour.
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ARG63212 anti-KLF15 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63212 anti-KLF15 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
